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Wheat-CCSHAU-Hisar
F4 generation
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CCSHAU, Hisar Breeding for high Fe and Zn

PI: Dr S.K. Sethi

Co PI: Dr Vikram Singh

Development of breeding material:

 

  • Biofortified Genotypes - WH 1080, WH 1063, WH 1127, WH 1136 (4)
  • High Yielding Genotypes - WH 1142, HD 3086, DPW 621-50, DBW 88, WH 1105, WH 542 (6)

 

WH 1080

PRL/2*PASTOR

WH 1063

BARBET 1 Selection

WH 1127

RL6043/4/NAC//PASTOR/3/BABAX

WH 1136

NI 5663/RAJ 3765

 

 

WH 1142

CHEN AEGILOPS SQUARROSA(TAUS)//FCT/3/2*WEAVER

HD 3086

DBW 14/HD 2733// HUW 468

DPW 621-50

KAUZ//ALTAR84/AOS/3/MILAN/KAUZ/4/HUITES

DBW 88

KAUZ//ALTAR84/AOS/3/MILAN/KAUZ/4/HUITES

WH 1105

MILAN/S87230//BABAX

WH 542

JUP/BJY“S”//URES



Molecular characterization of parents: Forty-nine microsatellite markers linked with identified QTL for micronutrient were used for screening the parents. Out of 49, 30 SSRs were found polymorphic which amplified a total of 76 alleles with an average of 2.00 alleles per locus. The amplified PCR products ranged from 100-480bp.

 

 

 

Electrophoretic pattern of biofortified bread wheat parents and F1s in SSR analysis using primer xbarc 220, Lane 1- M-Ladder, G1-HD3086, G2-HD2967, G3-DBW88, G4-WH1080, G5-WH1063, G6-WH1142, G7-WH1127, G8-WH1136, G9-WH542, G10-DPW621-50, G11-WH1105 

Using various combinations of crosses involving four biofortified genotypes and six high yielding genotypes, the following breeding material is being evaluated. Single ear selection in F4, back cross (BC1F3) and three way crosses (F3) generations will be done in 2018-19. 



 

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NIANP/NIN