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Wheat IIWBR
Molecular understanding of Fe and Zn
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ICAR-IIWBR, Karnal

PI: Dr Sewa Ram

Co PIs: Drs Sneh Narwal; O.P. Gupta, Vanita Pandey and Vikas Gupta

Development of RILs of HD 2967 and Glu-B1 line (1660): Using the Gpc-B1 lines provided by PAU, Ludihana, crosses were made with high yielding variety as HD 2967 for developing RILs. The analysis of F6 material showed large variations in grain protein, Fe and Zn contents. In addition, several backcross generations have also been developed with DPW 621-50 and DBW 88. Various combinations of reported markers viz., XCUW108 and 109 and CAPS marker XUHW 106, 86 and 84 were evaluated to identify Gpc-B1 in hexaploid backgrounds and as per the marker data generated, no correlation between Gpc-B1and grain protein content was observed. Therefore, efforts are being made to identify molecular marker associated with high grain protein content in RIL of HD 2967 and Gpc-B1 line.

 

Association mapping: QTL associated with high Fe and Zn content were identified using more than 16000 SNP data of 96 diverse wheat genotypes. 30 QTL with Fe content and 15 QTL for Zn content showed significant association across different chromosomes. 

   

Assessing variability in phytase and phytic acid levels in wheat varieties: Three hundred released wheat varieties of India were analysed for their variability in phytase and phytate levels using Direct assay method developed in IIWBR laboratory. The extraction and assay of the enzyme was faster and cost effective as compared to other methods. There were 6 fold variations in phytase levels and variation of 0.90 to 1.98% of phytic content among the varieties. Though, there was significant effect of both genotype and environment on phytate and phytase levels, higher heritability values (0.96 and 0.92 for phytase and phytate) suggested the potential of improving phytase levels through breeding.

 

 

Development and identification of mutant for high phytase and low phytic acid levels: More than 400 lines of mutants developed in the background of PBW 502 using EMS mutagenesis were evaluated for phytic acid and phytase levels using microlevel tests developed in IIWBR laboratory. Many of the lines showed very high phytase levels up to 2400 FTU/kg, while PBW 502 exhibited 720 FTU/kg. All the lines were evaluated for hectolitre weight for identification of lines with sound grains for further use in crossing with high yielding varieties. 

 

 



 

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